pE-100

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There are three standard pE-100 configuration options:

Direct-fit

(pE-100) for connecting to a microscope by selecting from a range of microscope adaptors which covers all current and most older models. A simple once only adjustment will allow optimisation to the optical path of the microscope.

Liquid Light Guide

(pE-100^{light guide}) with a fixed 3mm diameter, liquid light guide

Fiber

(pE-100^fiber) with an SMA connection for accepting multimode fibers. The pE-100^fiber has been designed with efficient coupling into a wide range of multimode fibers. The pE-100^fiber also includes excitation filter holders. Combining pE-100s:

Direct fit configuration

pE-100 units can be combined using the pE-Combiner for applications requiring a second LED wavelength with independent control and triggering.

Liquid light guide or multimode fiber

using a fixed two wavelength configuration can be specified. Two pE-100 Light Sources are combined, providing independent control and triggering.

The pE-100 offers:-

• Broadest LED spectrum available: 365-770nm – replaces metal halide or mercury

• Compatible with existing single and multi-band filter sets – no need to buy new filters

• Instant on/off - no shutters required, no warm up or cool down

• Simple to fit, simple to use – no alignment, a once only adjustment

• Stable & repeatable – reliable and consistent results

• Wide range of microscope adaptors – fits most microscopes

• Precise intensity control in 1% steps (0-100%) – no ND filters required

• Excellent uniformity over field of view – fixed and stable, no alignment necessary

• Long lifetime – expected to exceed 25,000 hours of operating time

Power requirements

100-240V a.c. 50/60Hz, 0.7A

Power consumption

2.2W (standby) 25W (full intensity)

Dimensions

pE-100 Light Source (direct fit): 60mm(w) x 170mm(d) x 70mm(h) - Weight 0.42kg pE-100^{light guide} Light Source: 52mm(w) x 95mm(d) x 52mm(h) – Weight 0.42kg pE-100^fiber Light Source: 76mm(w) x 165mm(d) x 80mm(h) - Weight 0.42kg Combined 2 x pE-100^fiber: 190mm(w) x 220mm(d) x 190mm (h) – Weight 1.5kg pE-100 Control Pod: 102mm(w) x 110mm(d) x 50mm(h) - Weight 0.55kg pE-100 Power Supply: 55mm(w) x 95mm(d) x 40mm(h) – Weight 0.19kg

Environment & Safety

LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

• Mercury-free

• Energy Efficient: 80% less power

• Long lifetime (25,000 operating hours)

• No bulb replacements

• Reduced risk of eye damage

• Quiet operation

• No special disposal regulations

Warranty

System = 24 months extendable by free product registration. LEDs = 36 months

Control pod for operation and access to settings and usage history.

Manual:

Manual control for instant on/off and intensity control in 1% steps from 0 – 100%

Remote:

Via single TTL for on/off control using a BNC connection on the control pod. Triggering speed approx. 150us

Ordering

244-87-XXX-YYY-ZZ:

pE-100 direct fit Light Source, fixed collimating adaptor to customer-specified microscope, remote manual control pod, and power supply

244-87-XXX-L15-ZZ:

pE-100^{light guide} Light Source with fixed 3mm diameter, 1.5m long liquid light guide, remote manual control pod, and power supply

pE-100-F-XXX-ZZ:

pE-100^fiber Light Source, remote manual control pod, SMA fiber connection, and power supply (no fiber provided)

pE-100-F-XXX-XXX-ZZ:

Combination of two pE-100^fiber Light Sources, remote manual control pod, SMA fiber connection, and power supply. Accepts 25mm diameter excitation filters (fiber and filters not provided)

A range of fibers is available from CoolLED. See Accessories

To specify wavelength (XXX) see LED Wavelengths To specify microscope code (YYY) see Adaptors

To specify power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA

Accessories

pE-10400: pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor

pE-ADAPTOR-YYY : Microscope adaptor to customer-specified microscope

To specify wavelength (XXX) see LED Wavelengths

To specify microscope code (YYY) see Adaptors

To specify power cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA

Fibers CoolLED’s pE-100 illumination system can be configured to accept a fiber via either an SMA or FC connector for connecting a wide range of fibers. The user can source the fiber independently or request supply from CoolLED by specifying:- Diameter in um (AAAA) Numerical Aperture in mm (0BB) Length in metres (C) Termination 1 i.e. SMA, FC or C60- cleaved 60mm bare (DDD) Termination 2 i.e. SMA, FC or C60-cleaved 60mm bare (EEE)

Product Code: pE-FAAAA-0BB-C-DDD-EEE A range of fibers is available from CoolLED. See Accessories

Examples below:-

pE-F50-022-2-SMA-SMA : 50 um diameter, 0.22 Numerical Aperture (NA), 2m long fiber with two SMA connections

pE-F50-022-2-SMA-C60 : 50 um diameter, 0.22 Numerical Aperture (NA), 2m long fiber with one SMA connection and one 60mm cleaved end

Contact CoolLED for your specific requirements

Reviews

"The main benefit of CoolLED's light source is the ability to switch on and off when required and also not having to align or change a bulb every 300 hours. Over the years it will pay for itself when compared to the cost of mercury bulbs."

Kevin MacKenzie, Histology/EM and Imaging Facility, Institute of Medical Sciences, Aberdeen University

"We have had great results using the CoolLED pE-100 on our TB, fungus and bacteria screening microscopes and find the CoolLED LED products make screening quick and easy"

Richard Wills, Microbiology Laboratory Manager at The Royal Berkshire Hospital

"I originally approached various companies with regards to getting an LED light source for my Leica DMLB microscope. The fact that the microscope was old and had a mechanized stage meant that some of the LED systems that we looked at were unsuitable to be fitted. Thankfully the CoolLED system simply slots in where the old mercury bulb unit used to sit. It took less than 5 minutes to fit, and was ready to use once it was switched on. No more waiting for the bulb to get to temperature, no more bulb changing, or re-calibrating of the mirrors before use. Simply marvellous!"

Kam Yim Wong, Immunology Dept, Derriford Hospital, Plymouth UK

CoolLED pE-100 (470 nm) provides very bright and stable excitation light for GCaMP3 imaging purpose. Usage of LED lamp s very easy and convenient, additionally TTL trigger on and off provides some flexibilities.

Chuanqiang Zhang, PhD student, Hans-Berger Department of Neurology, University Hospital Jena