PCR

Filters for Polymerase Chain Reaction (PCR)

With our line of PCR Sputtered optical filters, Chroma Technology supplies key components of polymerase chain reaction (PCR) laboratory and clinical test solutions, including digital PCR (ddPCR) and quantitative PCR (qPCR) platforms.


Below is an example of two different detection schemes used to detect the same four commonly used PCR fluorophores: FAM, HEX, ROX, and Cy5. The first scheme uses four single-band filter sets, each one optimized for a particular LED/fluorophore combination. The second uses one multi-band filter set with minimal filters and space requirements, but with compromised spectral efficiency. Other detection schemes may require only two fluorophores, while some may require six or more fluorescence channels.

A: Four individual narrow-band filter sets to detect nucleotides conjugated to FAM, HEX, ROX, or Cy5. Optimized for use with LEDs with output in the spectral regions of 465–475 nm, 525–535 nm, 560–580 nm, and 625–635 nm.

B: One single four-band filter set to detect nucleotides conjugated to FAM, HEX, ROX, or Cy5. Optimized for use with LEDs with output in the spectral regions of 465–475 nm, 520–535 nm, 580–595 nm, and 635–645 nm.

In both graphs, light gray shaded plots = excitation filter spectra, dark gray shaded plots = emission filter spectra, thick black traces = dichroic beamsplitter spectra.

Recommended filter set for detecting FAM in PCR instruments with an LED light source with output in the range of 455-485nm 

49401 

FAM Filter set #1 for PCR with LED


PCR470/30x (EX) 

PCR505lpxr (BS) 

PCR520/20m (EM) 

Recommended filter set for detecting HEX in PCR instruments with an LED light source with output in the range of 520-540nm 

49402 

HEX Filter set #1 for PCR with LED


PCR530/20x (EX) 

PCR550lpxr (BS) 

PCR565/22m (EM) 

Recommended filter set for detecting ROX in PCR instruments with an LED light source with output in the range of 560-580nm 

49403 

ROX Filter set #1 for PCR with LED


PCR570/20x (EX) 

PCR590lpxr (BS) 

PCR615/40m (EM) 

Recommended filter set for detecting Cy5 in PCR instruments with an LED light source with output in the range of 620-640nm 

49404 

Cy5 Filter set #1 for PCR with LED


PCR630/20x (EX) 

PCR647lpxr (BS) 

PCR665/20m (EM)