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BTX ECM 2001 ELECTROFUSION & ELECTROPORATION SYSTEMS

Get a multifunctional electrofusion and square wave electroporation generator that meets your exact needs. Available product configurations by application, such as embryo manipulation or hybridoma production, make choosing the right system easy.

For the ultimate in performance and convenience the ECM 2001+ offers advanced programming functions, preset protocols, and the most experimental flexibility.

For a reliable, affordable and easy-to-use cell fusion and mammalian transfection system, the ECM 2001 LITE may be just the right choice for you!

ECM 2001 SERIES GENERATORS

ECM 2001+ and ECM 2001 LITE are multifunctional electrofusion and square wave electroporation generators. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. These systems are powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

Waveforms

AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.

ECM 2001+ ELECTROFUSION AND ELECTROPORATION SYSTEM

The ECM 2001+ Electrofusion and Electroporation System provides the highest performance and flexibility for cell fusion applications such as hybridoma production, hybrid cell formation, embryo manipulation, nuclear transfer and transgenic applications. Additionally, square wave electroporation functions offer an efficient, reproducible solution for transfecting mammalian cells and tissues with DNA, mRNA, siRNA, proteins, drugs, CRISPR gene editing constructs and nanoparticles.

Features & Benefits

• Separate Electrofusion and Electroporation functional menus for streamlined protocol programming, data organization and navigation

• Advanced User-Defined Electrofusion protocols enable users to independently combine up to 19 AC protocol steps and up 1 DC protocol step in any order, for the highest performance and flexibility

• Convenient Pre-Set Electrofusion protocols for common hybridoma, embryo manipulation, and nuclear transfer applications

• Electrofusion and Electroporation Quick Start menus for speedy access to most used 3-step electrofusion and most used 1-step electroporation parameters

• Storage of up to 1,000 advanced User-Defined protocols

ECM 2001 LITE ELECTROFUSION AND ELECTROPORATION SYSTEM

The ECM 2001 LITE Electrofusion and Electroporation System offers a simple, effective option for cell fusion and square wave electroporation. The Quick Start Electrofusion menu offers a preconfigured 3-step protocol consisting of AC alignment, DC square wave, and AC. 

 Features & Benefits

• Quick Start Electrofusion menu with 3-step AC-DC-AC protocol structure for speedy access to the most used experimental parameters

• Storage of up to 1,000 User-Defined protocols with 3-step AC-DC-AC protocol structure

• Flexibility to inactivate the Electrofusion protocol AC steps for use in square wave electroporation applications, or to inactivate the Electrofusion protocol DC step for embryo manipulation applications

APPLICATIONS

Electrofusion

Fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications are facilitated by the combination of AC sine wave and DC square wave pulses. Fusion is achieved by the generation of an AC current sine waveform that generates a benign dielectrophoretic alignment of cells. Next, a DC square waveform is applied to promote fusion between aligned cells. After fusion, the AC sine waveform is reapplied maintaining the cell compression for the rounding off process resulting in a higher number of hybrids.

Mammalian Cell Transfection

Square wave electroporation is a highly flexible technique used to modify mammalian cells. Whether you are regulating gene expression up or down, expressing recombinant proteins, delivering non-cell-permeant molecules, or creating genetically modified model systems, electroporation is an ideal delivery method for all types of nucleic acids, proteins, drugs, and nanoparticles.

This method is non-toxic and requires no expensive reagents to successfully transfect your cells. Alternatively, adding additional selection steps to isolate stably transfected cells allows for integration of a gene into the genome of the cell for long term expression of protein.

The use of ECM 2001 series generators offers the control needed to adjust electrical settings for optimization of parameters. These systems are powerful enough to yield high transfection efficiencies and great survival rates for cell lines and difficult to transfect cell types including stem cells and primary cells.

In Vivo, In Utero & In Ovo Transfection

Square wave systems allow researchers to set the pulse lengths and number of pulses, which is critical to ensure viable cells and tissues while still maintaining efficient transfection both in vivo and ex vivo. Electroporation-mediated gene and drug delivery has been shown to substantially increase intracellular uptake and expression of DNA, siRNA and miRNA in muscle, skin, liver, kidney, testis, retina, tumors, etc. In vivo electroporation has successfully been used in embryo applications, in utero and in ovo applications, and in mammals, as well as other classical model systems such as chicken eggs, insects, and zebra fish.

PRODUCT CONFIGURATIONS BY APPLICATION

Complete Cell Fusion and Electroporation System

For use in a wide range of cell fusion applications and mammalian cell electroporation, ECM 2001 Cell Fusion Systems (45-2045, 45-2065) are our most versatile instrument configurations. The ECM 2001 Cell Fusion System comes with a selection of fusion chambers (Microslides, Meander Fusion Chamber, Flat Electrode) as well as safety stand and cuvettes for electroporation.

Mammalian Cell Transfections

For mammalian cell electroporation, ECM 2001 Electroporation Systems (45-2046, 45-2066) come with a safety stand and cuvettes for small volume in vitro electroporation. ECM 2001 Electroporation Systems with Monitoring (45-2050, 45-2070) additionally include a high voltage probe, connection box and oscilloscope for precise wave form monitoring and quality control. Combine Electroporation Systems with electrodes and accessories available separately for large volume in vitro electroporation, in vivo electroporation, adherent electroporation, or cell fusion applications.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In these systems the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Hybridoma Production

For use in large volume cell fusion applications such as hybridoma production, ECM 2001 generator, 2 ml optimization coaxial chamber, and 9 ml production coaxial chamber are used. These systems (45-2048, 45-2068) do not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. Coaxial chambers (47-0030, 47-0020) are also available for purchase separately.

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001 Embryo Manipulation Systems (45-2047, 45-2067). In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

Adherent Cell Transfections

Electroporate adherent cells directly into the dish used for cell growth. The ECM 2001 series generators coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

Build Your Own Custom System

Combine ECM 2001 Generators (45-2049, 45-2069) with separately available BTX electrofusion and electroporation electrodes and accessories to tailor the system to your experimental needs.