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pE-800fura (CoolLED)
pE-800fura (CoolLED)
Overview
Overview
Building on the successful pE-800 Illumination System, the pE-800fura offers the most comprehensive calcium imaging system available. It also enhances a range of applications from pH monitoring and optogenetics to everyday fluorescence microscopy, combining lightning-fast switching with eight individually controllable channels. Backed by CoolLED’s world-renowned support and generous warranty, the pE-800fura presents the go-to illuminator for calcium imaging and beyond.
Description
True 8-channel illumination
Eight powerful and efficient LEDs offer spectral coverage from 340-635 nm, tailored to all popular calcium indicators, pH indicators, opsins, as well as fluorophores for everyday fluorescence ranging from Fura-2 to Cy5.
Individually controlling all eight channels is achieved via USB, TTL or analogue for easily matching each LED to the selected calcium indicator or fluorophore set.
Unlike lasers, the broader spectral output of LEDs can be refined by the use of optical filters in order to excite a range of dyes, expanding the coverage of the pE-800fura. The ability to customise the wavelength output with both wavelength switching and optical filters means experiments are no longer limited by the light source, even as requirements evolve or new dyes emerge in the future.
Offering high-speed wavelength selection across the spectrum, the pE-800fura presents the first direct LED upgrade to traditional monochromator solutions with all the benefits of LED illumination.
We’ve got you covered: Unlike narrow-band illuminators such as lasers, broader LED wavelengths can be optimised with optical filters to excite a range of fluorophores. Suitable LEDs are shown for each fluorophore below, and we can recommend the best optical filters.
Accelerating calcium analysis
Industry-leading <7 µs TTL triggering is a breakthrough for ratiometric fluorescence applications such as calcium measurements, maximising the accuracy of measuring ratio pairs. For live cell imaging, capturing dynamic events is possible with the highest temporal resolution and even without software, thanks to precise hardware synchronisation via TTL input for each channel and compatibility with the pE-6501-8-FR (USB controlled TTL trigger box).
Even optical filtering is optimised for speed, with the simplicity of fitting single-band excitation filters at each LED channel avoiding the use of moving parts.
When used alongside multi-band filters, for example in a Pinkel configuration, this removes the need for slow and expensive external filter wheels and presents a low-cost approach to high-speed imaging.
Affordable automation with Sequence Runner
Taking speed one step further, Sequence Runner mode combines the versatility of software with the <7 µs speeds of TTL, with minimal requirement for expensive electronic hardware. All you need is a TTL-out. Once an LED and irradiance sequence are specified in software, Sequence Runner provides high-speed synchronised triggering via the global TTL-in of the pE-800fura and a single TTL-out from a camera (or other hardware).
When combined with inline filters, the pE-800fura transforms a manual microscope into an affordable and powerful eight-channel automated system for calcium imaging, ratiometric assays and everyday fluorescence.
Protecting live samples
Photodamage can have a serious impact on live cell data, and the pE-800fura has been carefully designed to overcome this, protecting samples and helping researchers generate the highest quality data.
Industry-leading <7 µs hardware synchronisation protects against photobleaching and phototoxicity by restricting sample exposure to image acquisition. Balancing irradiance to the lowest level possible while still maintaining image quality is another approach for reducing photodamage, and the pE-800fura simplifies this with fine irradiance control from 0-2%, and in 1% steps up to 100%.
Not everyone realises that excitation filters can still leak unwanted light, reducing the signal-to-noise ratio. The ability to select a single LED channel overcomes this, and has the added benefit of allowing a lower concentration of dyes such as Fura-2, which improves cell health and viability due to reduced toxicity – and also reduces reagent cost.
These factors not only improve data quality, but also push the boundaries of time-lapse studies.
Intuitive software control
Software control is achieved through a fast USB 2 and RS-232 connection and the user-friendly LightBridge Graphical User Interface (GUI), where control options include:
On/off control
LED selection
Real time irradiance control
Save and load pre-sets
pE-800fura start-up settings
Analogue settings
Ideal for optogenetics and electrophysiology
Eight individual analogue inputs allow automated high-speed irradiance control from 0-100% for each channel via analogue signal (0-10 V). This is ideal for many fluorescence applications as well as electrophysiology and optogenetics.
High Irradiance, Low Energy
The laser-free pE-800fura Illumination System builds on award-winning CoolLED technology, with stable and reliable performance and ultra-lower power consumption at 127 W. The sustainability benefits go beyond energy efficiency, and by removing the need for toxic mercury, the pE-800fura is a natural choice for cleaner, greener fluorescence microscopy.
Fit to your microscope
The compact system is compatible with the majority of microscope models. Liquid light guide delivery offers flexible placement.
Features
Broad spectrum from 340-635 nm tailored to popular calcium indicators and fluorophores
8 selectable channels offer versatility with any channels selectable simultaneously at 100%
Industry-leading <7 µs TTL triggering for high-speed analysis
Affordable automation with Sequence Runner and a single TTL-out
Removable inline excitation filter holder: no moving parts for high-speed imaging
Software (USB and RS-232), TTL, and analogue control options for optimised illumination
Optimised irradiance control in 1% steps (2-100%) and fine control from 0-2%
Light delivery via liquid Light Guide for maximum compatibility
Stable illumination minimises noise for precise calcium analysis and reduced false positives
High signal-to-noise for crisp images and quality data with less calcium indicator, reducing toxicity and cost
UV optimised optics maximises irradiance for Fura-2 and similar indicators
Plus all the benefits afforded by LED Illumination Systems, such as sustainability and longevity.
Ordering
pE-800-FR-L-SYS-ZZ:
pE-800fura Fluorescence Illumination System. For use with 3 mm Liquid Light Guide. Includes Light Source, Power Supply, Excitation Filter Holder (holds up to 8 filters), Excitation filters for 340 nm & 380 nm, USB cable & ZZ Plug
pE-1906:
1.5 m long, 3 mm diameter liquid light guide
pE-1908:
3 m long, 3 mm diameter liquid light guide
pE-340-FR-COLL-YYY:
pE-UV Universal Collimator & customer specified adaptor.
pE-CABLE-9WBNC-FR:
BNC Breakout Cable – 15 pin male connector to 9 x BNC female. Suitable for analogue or digital
pE-CABLE-9WSMB-FR:
SMB Breakout Cable – 15 pin male connector to 9 x SMB female. Suitable for analogue or digital
pE-6501-8-FR:
USB-TTL Conversion Kit for TTL control of pE-800fura with additional BNC for other peripherals (e.g. pT-100 for transmitted light). Connects to PC via USB and is compatible with range of imaging software.
pE-6501-8-FRS:
USB-TTL Conversion Kit when purchased with a pE-800fura Illumination System
pE-CABLE-15WHD2M:
Nikon communication cable for NiDAQ
To specify microscope code (YYY), see Adaptors
To specify Power Cable (20)
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