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Filters for Polymerase Chain Reaction (PCR)

With our line of PCR Sputtered optical filters, Chroma Technology supplies key components of polymerase chain reaction (PCR) laboratory and clinical test solutions, including digital PCR (ddPCR) and quantitative PCR (qPCR) platforms.

Below is an example of two different detection schemes used to detect the same four commonly used PCR fluorophores: FAM, HEX, ROX, and Cy5. The first scheme uses four single-band filter sets, each one optimized for a particular LED/fluorophore combination. The second uses one multi-band filter set with minimal filters and space requirements, but with compromised spectral efficiency. Other detection schemes may require only two fluorophores, while some may require six or more fluorescence channels.