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BTX GEMINI TWIN WAVE ELECTROPORATORS

The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.

These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.

FEATURES & BENEFITS

• Square wave and exponential decay waves power in a single unit

• Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)

• Large 7-inch, easy-to-use touch screen interface

• Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms

• Preset protocols – includes the most common eukaryotic and prokaryotic cell types

• User-defined protocols – unlimited ability to add and modify protocols

• Safety – displays resistance measurements for each pulse with three layers of arc protection

• Data management – stores logs of every pulse delivered for QC and troubleshooting

GEMINI SC SYSTEM

The Gemini SC system is designed specifically for cuvette-based in vitro transfections of eukaryotic or prokaryotic cells in suspension. With a wide range of pulsing parameters, advanced safety features as well as dozens of preset protocols, the Gemini SC is ideal for any lab requiring efficient cell transfection or transformation without the use of costly reagents.

GEMINI X2 AND GEMINI X2 HT and GEMINI X2 PULSE SWITCHER SYSTEMS

The Gemini X2 system provides the ultimate experimental flexibility. This one easy setup allows for transfections in cuvettes or high throughput plates, or the capability to utilize the wide variety of BTX specialty electrodes for specific applications. In addition, Gemini X2 electrical output specifications are wide-ranging, making it the most versatile electroporation system available today.

Electroporation of suspension cells can be achieved in cuvettes (X2) or 96-well plates (X2 HT) with an HT plate handler. Additionally, the Gemini X2 can be paired with BTX specialty electrodes to deliver gene and drugs in vivo, in utero, in ovo and to explant tissues as well as adherent cells.

Combine the Gemini X2 with the Pulse Switcher to incorporate automatic bipolar switching into electroporation protocols. Polarity switching enhances transfection efficiency by preventing transfectant accumulation at a single electrode and enhancing delivery to the target cells or tissue.

GEMINI'S BROAD CAPABILITIES

Exponential Decay/Square Twin Waveforms

Allows researchers to select which type of waveform they would like to use to transfect eukaryotic cells or transform prokaryotic cells. Typically, eukaryotic cells respond best to square wave pulses while prokaryotic cell respond well to exponential decay wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their application.

Simple User Interface

Permits intuitive touch screen or computer-controlled protocol programing and execution as well as password protection of protocols.Each system is programmable and offers unlimited protocol storage as well as dozens of proven pre-set protocols to reduce optimization time. The X2 systems provide protocol delivery log storage. Logs can be accessed for quality control, optimization or troubleshooting purposes.

Pre-Pulse Resistance Measurement

Offers assurance that sample resistance is within acceptable range for electroporation. This feature protects precious sample as well as system component to safeguard against arcing.

Widest Voltage/Pulse length Range Available

Provides researchers with the widest range of voltage options available in the market.

• The Gemini X2 can achieve voltage delivery as low as 5 V and as high as 3000 V in both waveforms in addition to pulse lengths of 10 µs to 5 s, from delicate in ovo tissue to hearty gram positive bacteria.

• The Gemini SC can achieve voltage delivery as low as 10 V and as high as 3000 V in both waveforms in addition to pulse lengths of 50 µs to 5 s.

Highly Accurate Pulsing

Delivers precise voltage, pulse length/time constant, pulse interval and displays the values back to the researcher allowing for visual confirmation of successful electroporation.

Revolutionary Arc Proofing Safety Features

Gives researchers peace of mind knowing that their sample and their systems are safe from troublesome arcing with enhanced 4-way safety checks through the pulsing procedure. Using two separate pre pulse, sample resistance monitoring scans to ensure proper conditions, as well as internal safety features auditing pulse progress in at the component as well as software level, arcing is dramatically decreased.

The Gemini is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

INCLUDED ITEMS

Item #

Description

Included Items

45-2040

Gemini X2 Electroporation System Complete for Cuvettes

Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and Cuvette Rack 660

45-2041

Gemini X2 Electroporation Generator Only

Gemini X2 Electroporation Generator only

45-2042

Gemini SC Electroporation System Complete for Cuvettes

Gemini SC Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. 30 (10 each), and Cuvette Rack 660

45-2043

Gemini SC Electroporation Generator Only

Gemini SC Electroporation Generator only

45-2044

Gemini X2 High Throughput System, 96-well with HT-200

Gemini X2 Generator, Safety Dome, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each), and cuvette Rack 660, HT-200 Plate Handler, 2 mm gap HT plate and 4 mm gap HT plate

 45-2062

 Gemini X2 Pulse Switcher Bipolar Electroporation System

Gemini X2 Generator, Pulse Switcher, Power Supply and USB connector cable, Safety Dome, Cuvettes 1 mm, 2 mm,4 mm (pkg. of 30, 10 each), and Cuvette Rack

GEMINI X2 SPECIFICATIONS

SQUARE WAVE PULSE RANGES 

Voltage Range 

  LV Mode : 5 to 500 V in 1 V steps

  HV Mode : 505 to 3000 V in 5 V steps

Voltage Accuracy 

  LV Mode : 5%

  HV Mode : 5%

Pulse Length Range 

  LV Mode : 10 μs to 999 μs in 1 μs steps

                         1 ms to 999 ms in 1 ms steps

  HV Mode : 10 μs to 600 μs in 1 μs steps

Multiple Pulsing 

  Single Samples : 1 to 99 pulses per sample

  HT Plate Handler Mode : 1 to 10 pulses per sample (up to 120 total)

  Pulse Interval : 0.1 s to 10 s

Capacitance 

  LV Mode : 3775 μF

  HV Mode : 85 μF

  Voltage Droop : 20% Load <1 K Ω, 10% Load >1 K Ω

EXPONENTIAL DECAY PULSE RANGES 

Voltage Range

   LV Mode : 5 to 500 in 1 V steps

  HV Mode ; 505 to 3000 in 5 V steps

Voltage Accuracy 

  LV Mode : 5%

  HV Mode : 5%

Time Constant Range 

  LV Mode : 1 ms to 5.158 s

  HV Mode : 0.5 ms to 133.875 ms

Multiple Pulsing 

  Multiple Pulsing : 1 to 2 depending on device's current amplitude limit

  Pulse Interval : 5 to 30 s

Capacitance 

   LV Mode : 25 to 3275 μF in 25 μF steps

  HV Mode : 10, 25, 35, 50, 60, 75, 85 μF

Available Internal Resistance Selections 

  All Modes : LV 25 to 1575 Ω in 25 Ω steps

                          HV 50 to1575 Ω in 25 Ω steps

SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE 

LV Mode : PL ≤100 ms, load ≥8 to 9 Ω, PL >100 ms, load ≥100 Ω

HV Mode : Load ≥40 Ω

GEMINI SC SPECIFICATIONS

SQUARE WAVE PULSE RANGES 

Voltage Range 

  LV Mode : 10 to 500 V in 5 V steps

  HV Mode : 510 to 3000 V in 10 V steps

Voltage Accuracy 

  LV Mode : 5%

  HV Mode : 5%

Pulse Length Range 

  LV Mode : 0.05 ms to 10 ms in 0.05 ms steps

                         10 ms to 100 ms in 1 ms steps

  HV Mode : 50 μs to 5 ms in 50 μs steps

Multiple Pulsing 

  LV Mode : 1 to 10 pulses per sample

  HV Mode : 1 to 2 pulses per sample

  Pulse Interval :  0.1 s to 10 s

Capacitance 

  LV Mode : 3775 μF

  HV Mode : 85 μF

  Voltage Droop : 20% Load <1 K Ω, 10% Load >1 K Ω

EXPONENTIAL DECAY PULSE RANGES 

Voltage Range 

  LV Mode : 10 to 500 V in 5 V steps

  HV Mode : 510 to 3000 V in 10 V steps

Voltage Accuracy 

  LV Mode : 5%

  HV Mode : 5%

Time Constant Range 

  LV Mode : 1.25 ms to 3.275 s / 1.25 ms

  HV Mode : 0.5 ms to 50 ms / 0.5 ms

Multiple Pulsing 

  Multiple Pulsing : No

  Pulse Interval : N/A

Capacitance 

  LV Mode : 25 to 3275 μF in 25 μF steps

  HV Mode : 10, 25 and 50 μF

Available Internal Resistance Selections 

  All Modes : 50 to 1000 Ω in 50 Ω steps

SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE 

  LV Mode : Load ≥10 Ω

  HV Mode : 510 - 2500 V Load ≥20 Ω, 2500 - 3000 V, Load  ≥600 Ω