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pE-4000 (CoolLED)

The NEW Enhanced pE-4000 is a powerful 4-channel high-specification LED illumination system. It is flexible, controllable and environmentally friendly.  Benefits include:

SELECTABILITY

• Choice of 256 wavelength combinations from 16 installed LEDs (365nm -770nm)

• Choose 1 of 4 wavelengths for each 4 channels on the control pod

• Unique wavelength grouping concept (see below)

• Optimum wavelength to suit your experiment

• Specific wavelength characterisation for optimal optogenetic control of cell responses

Wavelength Grouping Concept

As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains.  These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available.  This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

SUSTAINABILITY

• Lowest Power Consumption – other LED technology uses 120-350 Watts

• >25000 hours operation

• Highly stable, repeatable LED technology

• Market leading energy efficiency

CELL VIABILITY

• Optimal illumination through control features to extend fluorescence of cells

• Reduced photobleaching and phototoxicity

• Microsecond switching

• Variable pulse duration on/off

“When you can only control intensity of ‘white’ light (rather than individual channels), the level of photobleaching can be high.  With the pE-4000, we can control the excitation of the individual channels.  It is possible to optimise the excitation intensity according to the labelling, greatly reducing photobleaching and phototoxicity in a live experiment.”    

Dr Yan Gu, University of Sussex

“Suddenly, we were able to offer users uninterrupted extended live cell experiments of 100+ hours, without worrying about brightness fluctuations, lamps burning, room heating, etc. Also, users have reported markedly reduced bleaching and phototoxicity in their samples, both from the prokaryotic and the eukaryotic research fields.” 

Dr Jens Eriksson, Oslo University Hospital

  VISIBILITY

• High signal to noise ratio which gives cleaner images and data

“Striking, bright fluorescence images with a strong signal to noise ratio even when using a low magnification objective” 

                          Graham Wright, Institute of Medical Biology, A*STAR Singapore

RELIABILITY

• Assured through experience and QA assured processes

• 3 year Warranty after registration

• First year Warranty swap

The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics.  A dual mode control pod gives manual control in either White or Advanced modes.  Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber.  LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range.

The pE-4000 offers:-

• Broadest LED spectrum available: 365-770nm – match LED peaks to existing microscope filters

• Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters

• Individual control of selected LED wavelengths – less background, no filter wheel or shutters

• Integrated with many imaging software packages - can be incorporated into all experimental parameters

• Instant on/off – no shutters required, no warm up or cool down

• Stable & repeatable – reliable and consistent results

• Wide range of Microscope Adaptors – fits most microscopes

• Precise intensity control in 1% steps (0-100%) – no ND filters required

• Excellent uniformity over field of view – fixed and stable, no alignment necessary

• Long lifetime – expected to exceed 25,000 hours of operating time

IDEAL FOR MULTI-USER AND CORE FACILITIES

• Pre-sets allowing lab manager to match white spectrum to existing filter cubes

• Higher contrast images from matched-white spectrum

• Simple to fit, simple to use

• No training required

IDEAL FOR ADVANCED RESEARCH

• Individual LED wavelength selection

• Rapid switching between LED wavelengths enables capture of high speed events

• TTL & USB interfaces with imaging packages

• Excitation filters can be fitted in optical path for controlled switching with no moving parts

• Analogue inputs for dynamic intensity control

• Optical feedback for applications requiring higher stability

• Internal function generator for electrophysiology and optogenetics applications

Power requirements

110-240V a.c. 50/60Hz, 2.5A

Power consumption

Standby (i.e. no LEDs on) : Max 7W 

Single wavelength operation : Max 25W 

Dual wavelength operation : Max 44W 

Triple wavelength operation : Max 53W 

Quad wavelength operation : Max 60W

Dimensions

pE-4000 Light Source: 150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg 

pE-4000 Control pod: 154mm(w) x 135mm(d) x 40mm(h) – Weight 0.95kg 

pE-4000 Power Supply: 164mm(w) x 64mm(d) x 35mm(h) – Weight 0.58kg 

pE-Expansion Box: 151mm(w) x 18mm(d) x 95mm(h) - Weight 0.34kg

Environment & Safety

LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

• Mercury-free & Laser-free

• Energy Efficient

• Long lifetime (25,000 operating hours)

• No bulb replacements

• Reduced risk of eye damage

• Quiet operation

• No special disposal regulations or issues

Warranty

System =24 months extendable by free product registration.  LEDs = 36 months

Manual : Dual function remote manual control pod for White mode or Advanced mode

Remote: Via USB for independent on/off and intensity control of each channel. Triggering speed approx 1ms 

Via 4 TTL inputs for independent on/off control of each channel. Triggering speed <20us 

Via single TTL for on/off control of manual or software selected channels 

Via 4 analogue inputs 0-10V, 0-300kHz for dynamic control of intensity from external analogue signals

Synch Out: 4 TTL outputs for each channel – active high 1 TTL output for any channel – active high

Programmable interface: 4 TTL outputs for on/off control of peripherals (transmitted illuminators, stages etc.) 4 analogue outputs for intensity control of peripherals (can be programmed to mirror LED intensities for channel control) 0-10V full scale.

Function Generator: Internally generated sine, pulse and ramps for each channel programmed via control pod.

Connectivity: USB (B type) for PC connection. All other TTL and Analogue inputs/outputs via 25way 'D-type' female connector (optional rear mounting pE-Expansion Box available for BNC connectivity).

Imaging Software: Recognised under common software e.g. Micromanager, MetaMorph, cellSens, NIS Elements, etc. see Imaging Software

Ordering

pE-4000-L-SYS-ZZ:   pE-4000 Light Source with manual control pod, and power supply for LLG delivery

pE-4000-F-SYS-ZZ:   pE-4000 Light Source with manual control pod and power supply for Fiber delivery

pE-4000-EB25D:        Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity

pE-4000-EFH-4          Set of four Excitation Filter holders (25mm dia.)

pE-1906:                    1.5m long, 3mm diameter liquid light guide

pE-10400:                  pE-Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor

pE-ADAPTOR-YYY   Microscope Adaptor to customer-specified microscope

A range of fibers is available from CoolLED, go to Accessories tab To specify microscope code (YYY), see Adaptors

To specify Power Cable (ZZ):  10 = Australia, 20 = Europe, 30 = UK, 40 = USA